Free dilution toolUpdated

Bacteriostatic water calculator.

Divide the vial's mg by the concentration you want in mg/mL. 2 mL in a 10 mg vial makes 5 mg/mL; 1 mL makes 10 mg/mL, where 10 units on a U-100 syringe is exactly 1 mg.

Dilution

Calculate how much bac water to add.

Compare a candidate water volume before it changes the concentration of the whole vial.

mg
mL
mcg
Draw to
10 units

10 mg in 1 mL · 100 mcg per unit

10 full doses per vial

0u10u drawn100u

On a 100 unit insulin syringe. The app remembers your setup so you never re-enter it.

How bacteriostatic water sets concentration.

The water never changes how much peptide is in the vial. It only changes how much liquid each dose sits in.

  1. 01

    Start with the vial strength

    The total amount on the label
  2. 02

    Try a water volume

    A candidate amount in millilitres
  3. 03

    Compare the resulting draw

    Concentration, volume, and units

What bacteriostatic water is

Bacteriostatic water is sterile water with 0.9% benzyl alcohol added as a preservative, as the FDA label on DailyMed states. The preservative is what allows a vial to be entered more than once, because it inhibits bacterial growth in the remaining solution. Plain sterile water has no preservative and is typically treated as single-use. The same label carries a warning against use in newborns, because benzyl alcohol can be toxic to them.

Bacteriostatic waterSterile water for injection
Preservative0.9% benzyl alcoholNone
Typical useMulti-dose vialsSingle use
Once openedUp to 28 days from first entry, unless the label says otherwiseDiscard after use
StoragePer the manufacturer labelPer the manufacturer label

Choosing a diluent volume

There is no single correct volume. It is a trade-off, and it is set with your healthcare professional. The mechanics, though, are fixed:

  • More water gives a lower concentration and a larger draw for the same dose. Larger draws are read against more of the barrel, which typically makes small measurement errors proportionally smaller.
  • Less water gives a higher concentration and a smaller draw. That keeps injection volume down, but a dose may land within the first few units of the barrel, where a half-unit misread is a larger share of the total.
  • Vial capacity is a hard limit. A 2 mL vial cannot take 3 mL of diluent, whatever the arithmetic says.

Reverse-calculating from a target concentration

To hit a specific concentration, divide the vial strength by it. For a 10 mg vial at a target of 5 mg/mL, that is 10 ÷ 5 = 2 mL of bacteriostatic water.

Diluent volume needed to reach a target concentration. Check each volume against the vial's own capacity: a 3 mL vial cannot take 5 mL or 7.5 mL, and no common vial holds the 15 mL that 30 mg at 2 mg/mL would need.

Vial strengthTarget 2 mg/mLTarget 5 mg/mLTarget 10 mg/mL
2 mg1 mL0.4 mL0.2 mL
5 mg2.5 mL1 mL0.5 mL
10 mg5 mL2 mL1 mL
15 mg7.5 mL3 mL1.5 mL
30 mg15 mL, too large6 mL3 mL

Units to draw on a U-100 syringe for an example 250 mcg dose. The mark depends only on the concentration, not the size of the vial.

ConcentrationDraw for 250 mcg
2 mg/mL12.5 units
5 mg/mL5 units
10 mg/mL2.5 units

The 2.5 unit draw is the one to look at twice. A 1 mL syringe is typically graduated every 2 units, so that mark falls between two lines, and reading it half a unit out is 20% of the dose. At 12.5 units the same slip is 4%. More water moves the same dose further up the barrel, and a 0.3 mL syringe, graduated every unit, reads a small draw more finely. Reading a U-100 insulin syringe covers both.

A convenient trick: add 1 mL of water. The concentration in mg/mL then equals the vial strength, and on a U-100 syringe 100 units is the whole vial, so 10 units is one-tenth of it. For any other dilution, the peptide reconstitution calculator turns the dose into the mark.

Bacteriostatic water FAQs.

The questions that come up before the water goes in: what the volume changes, and what it doesn't.

How much bacteriostatic water do I add to a peptide vial?

Divide the vial strength in mg by the concentration you are aiming for in mg/mL. A 5 mg vial at 2.5 mg/mL takes 2 mL; a 10 mg vial at 5 mg/mL takes 2 mL too. Which concentration to aim for is a decision for your healthcare professional, not a fixed number.

What is the difference between bacteriostatic and sterile water?

Bacteriostatic water contains around 0.9% benzyl alcohol as a preservative, which is what typically allows multi-dose use. Sterile water for injection has no preservative and is generally single use.

How long does a vial last after bacteriostatic water is added?

CDC guidance for multi-dose vials is to discard them within 28 days of first entry, unless the manufacturer states another date. The peptide itself has its own stability characteristics, which vary. Both are questions for your healthcare professional and the product labelling.

Does adding more water reduce the dose?

No. It reduces the concentration, so the same dose occupies a larger volume. The amount of peptide drawn is unchanged; only the amount of liquid carrying it changes.

Can I add too much bacteriostatic water?

The practical ceiling is the physical capacity of the vial. Beyond that, a very dilute solution means a large injection volume for each dose, which is a trade-off to discuss with your healthcare professional.